In 2014 she spend a year as a medical officer training in the Intensive Care Unit of the Chris Hani Baragwanath Hospital, followed by a year in the department of anaesthesia. She began her registrar training in 2016 in Anaesthesia in the Johannesburg circuit. After qualifying as a specialist through the College of Medicine of South Africa, she completed her Master of Medicine in Anaesthesia at the University of Witwatersrand.

With her expertise, Dr Sehlapelo has also been actively involved in the training and supervision of specialists-in-training and medical students, as well as participating in studies that were being carried out in the Department of Anaesthesia at the University. With extensive knowledge in the field of Anaesthesia, she works as a consultant Anaesthesiologist at Chris Hani Baragwanath Hospital, the third largest hospital in the world. While being actively involved in the tertiary hospital environment, anaesthetising a myriad of surgical conditions some of which are complex in nature referred from other hospitals, Dr Mathabe Sehlapelo has carved a niche for herself in the fields of neuroanaesthesia and airway management.